Journal: EMBO Reports

Article Title: Conserved lipid metabolic reprogramming confers hypoxic and aging resilience

doi: 10.1038/s44319-025-00664-6

Figure Lengend Snippet: ( A ) Schematic illustrating fatty acid synthesis and desaturation pathways. ( B ) Representative confocal fluorescence images showing the expression of FASN-1::GFP under normal or hypoxic (0.1%) conditions for 24 h post L4 stages. Scale bars: 100 µm (low mag) or 10 µm (high mag). ( C ) Representative confocal fluorescence images showing high-resolution Z-stack views of fat-6p::fat-6::GFP under normal or hypoxic (0.1%) conditions for 24 h post L4 stages. Scale bars: 10 µm. ( D ) Representative confocal fluorescence images showing low and high-resolution Z-stack views of fat-6p::fat-6::GFP under normal or hypoxic (0.5%) conditions for 24 h post L4 stages. Scale bars: 10 µm. ( E ) Quantification of the percentage of animals with fat-6p::fat-6::GFP fluorescence intensities downregulated under normal or hypoxic (0.5%) conditions for 24 h post L4 stages. Data were presented as means ± S.D. P values calculated by unpaired two-tailed t-tests. *** P < 0.001 (normal, n = 30 animals, hypoxic, n = 37 animals). ( F ) Representative confocal fluorescence images showing the downregulation of dhs-3p::dhs-3::GFP -labeled lipid droplet abundance and number under normal or hypoxic (0.1%) conditions for 24 h post L4 stages. Scale bars: 10 µm. ( G ) Representative confocal fluorescence images showing the expression of HIF-1-dependent transcriptional reporter, nis470 (cysl-2p::mVenus) abundance under DMSO or VHL-1 inhibitor VH-298 (1 mg/ml) for 96 h post embryos stage. Scale bars: 10 µm. ( H ) Representative confocal fluorescence images showing low and high-resolution Z-stack views of fat-6p::fat-6::GFP under DMSO or VHL-1 inhibitor VH-298 (1 mg/ml) for 96 h post embryos stage. Scale bars: 10 µm. ( I ) Quantification of the percentage of animals with fat-6p::fat-6::GFP fluorescence intensities downregulated under DMSO or VHL-1 inhibitor VH-298 (1 mg/ml) for 96 h post embryos stage. Data were presented as means ± SEM. P values calculated by unpaired two-tailed t-tests. * P < 0.05 ( n = 30 animals per condition). ( J ) Representative confocal fluorescence images showing fat-6p::fat-6::GFP animals fed with control RNAi or RNAi against hif-1 or vhl-1 under normal or hypoxic conditions. Scale bars: 10 µm. ( K ) Representative confocal fluorescence images showing dhs-3p::dhs-3::GFP animals fed with control RNAi or RNAi against hif-1 or vhl-1 under normal conditions. Scale bars: 10 µm. ( L ) Representative confocal fluorescence images showing FASN-1::GFP animals fed with control RNAi or RNAi against vhl-1 under normal conditions. Scale bars: 10 µm. .

Article Snippet: VH-298 , MedChemExpress , Cat# HY-100947.

Techniques: Fluorescence, Expressing, Two Tailed Test, Labeling, Control

Journal: EMBO Reports

Article Title: Conserved lipid metabolic reprogramming confers hypoxic and aging resilience

doi: 10.1038/s44319-025-00664-6

Figure Lengend Snippet: ( A ) Representative confocal fluorescence images showing the expression of an integrated transgene fat-6p::fat-6::GFP under normal conditions at L1-L4 stages. Scale bars: 100 µm. ( B ) Representative Low and high magnification confocal fluorescence images showing the expression of fat-6p::fat-6::GFP under normal conditions L4 stages. Scale bars: 10 µm. ( C ) Representative confocal fluorescence images showing low and high-resolution Z-stack views of fat-6p::fat-6::GFP under normal or hypoxic (5%) conditions for 24 h post L4 stages. Scale bars: 10 µm. ( D ) Quantification of the percentage of animals with fat-6p::fat-6::GFP fluorescence intensities downregulated under normal or hypoxic (5%) conditions for 24 h post L4 stages. Data were presented as means ± S.D. P values calculated by unpaired two-tailed t-tests. n.s indicates nonsignificant ( n > 25 animals per condition). ( E ) Representative confocal fluorescence images showing fat-6p::fat-6::GFP fed with control RNAi or RNAi against hif-1 . Scale bars: 10 µm. ( F ) Representative confocal fluorescence images showing the downregulation of dhs-3p::dhs-3::GFP -labeled lipid droplet number and size under normal or hypoxic (0.5%) conditions for 24 h post-L4 stages. Scale bars: 10 µm. ( G ) Schematic diagram of HIF-1, VHL-1, and the drug VH-298. Representative confocal fluorescence images showing the upregulation of HIF-1 target reporter cysl-2p::Venus treat with DMSO or VHL-1 inhibitor VH-298 (1 mg/ml) for 48 h starting at embryos. Scale bars: 10 µm. ( H ) Representative confocal fluorescence images showing the upregulation of HIF-1 target reporter cysl-2p::Venus treat with DMSO or VHL-1 inhibitor VH-298 (10 mg/ml) for 48 h starting at embryos. Scale bars: 10 µm. ( I ) Representative confocal fluorescence images showing the upregulation of HIF-1 target reporter cysl-2p::Venus treat with DMSO or VHL-1 inhibitor VH-298 (10 mg/ml) for 96 h starting at embryos. Scale bars: 10 µm.

Article Snippet: VH-298 , MedChemExpress , Cat# HY-100947.

Techniques: Fluorescence, Expressing, Two Tailed Test, Control, Labeling